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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Pesca e Aquicultura. |
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Data corrente: |
03/11/2021 |
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Data da última atualização: |
04/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ADOLFI, M. C.; DU, K.; KNEITZ, S.; CABAU, C.; ZAHM, M.; KLOPP, C.; FERON, R.; PAIXÃO, R. V.; VARELA, E. S.; O'SULLIVAN, F. L. A.; OLIVEIRA, M. A. de; NÓBREGA, R. H.; LOPEZ-ROQUES, C.; IAMPIETRO, C.; LLUCH, J.; KLOAS, W.; WUERTZ, S.; SCHAEFER, F.; STÖCK, M.; GUIGUEN, Y.; SCHARTL, M. |
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Afiliação: |
MATEUS C. ADOLFI, University of Wuerzburg; KANG DU, University of Wuerzburg; SUSANNE KNEITZ, University of Wuerzburg; CÉDRIC CABAU, Université de Toulouse; MARGOT ZAHM, Université de Toulouse; CHRISTOPHE KLOPP, INRA; ROMAIN FERON, INRAE; RÔMULO V. PAIXÃO, Bolsista CPAA; EDUARDO SOUSA VARELA, CNPASA; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; MARCOS A. DE OLIVEIRA, UNESP; RAFAEL H. NÓBREGA, UNESP; CÉLINE LOPEZ-ROQUES, INRAE; CAROLE IAMPIETRO, INRAE; JÉRÔME LLUCH, INRAE; WERNER KLOAS, Institute of Freshwater Ecology and Inland Fisheries; SVEN WUERTZ, Institute of Freshwater Ecology and Inland Fisheries; FABIAN SCHAEFER, Institute of Freshwater Ecology and Inland Fisheries; MATTHIAS STÖCK, Institute of Freshwater Ecology and Inland Fisheries; YANN GUIGUEN, INRAE; MANFRED SCHARTL, Comprehensive Cancer Center Mainfranken. |
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Título: |
A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas). |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientific Reports, v. 11, art. 21544, 2021. |
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ISSN: |
2045-2322 |
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DOI: |
https://doi.org/10.1038/s41598-021-01066-z |
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Idioma: |
Inglês |
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Conteúdo: |
Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF B signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes. |
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Thesagro: |
Cromossoma; Peixe; Pirarucu. |
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Thesaurus Nal: |
Arapaima gigas; Chromosomes; Cytogenetics; Fish; Sex chromosomes; Sex determination. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227368/1/Arapaima-sexmaster-gene-2021.pdf
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Marc: |
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001 2135766
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008 2021 bl uuuu u00u1 u #d
022 $a2045-2322
024 7 $ahttps://doi.org/10.1038/s41598-021-01066-z$2DOI
100 1 $aADOLFI, M. C.
245 $aA duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas).$h[electronic resource]
260 $c2021
520 $aArapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF B signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes.
650 $aArapaima gigas
650 $aChromosomes
650 $aCytogenetics
650 $aFish
650 $aSex chromosomes
650 $aSex determination
650 $aCromossoma
650 $aPeixe
650 $aPirarucu
700 1 $aDU, K.
700 1 $aKNEITZ, S.
700 1 $aCABAU, C.
700 1 $aZAHM, M.
700 1 $aKLOPP, C.
700 1 $aFERON, R.
700 1 $aPAIXÃO, R. V.
700 1 $aVARELA, E. S.
700 1 $aO'SULLIVAN, F. L. A.
700 1 $aOLIVEIRA, M. A. de
700 1 $aNÓBREGA, R. H.
700 1 $aLOPEZ-ROQUES, C.
700 1 $aIAMPIETRO, C.
700 1 $aLLUCH, J.
700 1 $aKLOAS, W.
700 1 $aWUERTZ, S.
700 1 $aSCHAEFER, F.
700 1 $aSTÖCK, M.
700 1 $aGUIGUEN, Y.
700 1 $aSCHARTL, M.
773 $tScientific Reports$gv. 11, art. 21544, 2021.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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